Phytochemical Screening of Mimosa pudicaaerial part and evaluation of itspotent antiulcereffect in terms of phenolic and flavonoid components

Abstract

Free radical’s toxic by-products of natural metabolism of a cell, are responsible for causing a wide number of health problems. Search for plant based dietary supplements or drugs are always in great demand. This is due to characteristic flavonoids and phenolicswith antioxidant, anti-lipoxygenase, anticancer etc. clinical activities. In the study presented, aerial parts hydro-alcoholic extracts of Mimosa pudica were used for in-vitro analysis of having antioxidant activity. Three separate assay methods were used to evaluate the antioxidant activity i.e. against DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical, total flavonoid and phenolic content were investigated. This was done by formulatingmethanolic extract (70/30 ethanol to water) of Mimosa pudicaand ascorbic acid as standard. % scavenging activity and IC50 value were measured for extract prepared at various concentrations. The results IC₅₀values was 46.58 µg/mlfor DPPHradicals. Subsequently,total phenolic and flavonoids content wereobtained in three different extracts. Namely, chloroform extract (CEMP), methanolic extract (MEMP), and diethyl ether extract (DEMP). The total phenolic content (TPC) for extracts i.e. CEMP, MEMP, and DEMP were 8.49 ± 1.05 mg, 15.74 ± 1.21 mg, and 11.64 ± 1.21 mg of GAE/100 gm, respectively. The total flavonoids content (TFC)for extracts namely CEMP, MEMP, and DEMP obtained was10.85 ± 0.11 mg, 14.41 ± 0.03 mg, and 9.98 ± 0.48 mg of QE/100 g, respectively.Occurrence,in the hydro-alcoholic extract, of a greater % of phyto-constituents of Mimosa pudica,put forward that it may holdmedicinal antioxidant properties. Overall, presented research work offers noteworthydonations and improvementsin thearena of phytomedicines, fostering innovation.