Histopathological Changes In Diabetic Nephropathy Treated With Fenugreek In Streptozotocin Induced Rat Models

Abstract

Background: DN is the primary cause of kidney diseases in patients requiring renal replacement therapy, with approximately 40% of these patients also experiencing diabetes. Research proves that fenugreek seed extractions possess antioxidant, hypocholesterolemic, and antidiabetic properties. This study was conducted to observe effect of fenugreek on Streptozotocin induced diabetic nephropathy and also to compare histopathological studies Methods:  This is an experimental study conducted among 12 rats (4 control rats, 4 diabetic rats, 4 diabetic rats treated with fenugreek) in an animal house belonging to pharmacology department of a tertiary care medical college situated in South India. Blood glucose control was assessed using serum blood glucose and HbA1c concentrations. The renal function was studied using dimethyl formamide (DMF), 24 h urinary albumin excretion (albuminuria), Blood Urine Nitrogen, Serum Creatinine and Kidney Index Results: There was significant difference in blood glucose levels, HbA1c concentration and renal function parameters among diabetic rats treated with fenugreek between both control group and diabetic control groups. PAS positive staining and glomerular was significantly low among DN Fenugreek group microscopy signifying the prevention of ECM accumulation when compared to both control and DN control group. Conclusion: Our study proves both protective effect as well as the significant histopathological changes of fenugreek on diabetic nephropathy.