Formulation, optimization and characterization of Mfx-Gold nanoparticle loaded in-situ gel for ophthalmic drug delivery using box-benken design approach
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Abstract
The aim of the present investigation is to prepare and evaluate Mfx-GNPs loaded in-situ gel-for ophthalmic drug delivery system. The study is proposed to evaluate the anti-bacterial and anti-biofilm efficacies of Mfx-GNPs loaded in-situ gel. This article describes a systematic approach for the design, optimization, and characterization of Mfx-GNPs loaded in-situ gel. Optimization was performed using a 32 response surface approach, the effect of Concentration of Gellan gum (X1), Concentration of Chitosan (X2) and Concentration of Carbopol 940p (X3) content was optimized as independent variables, Viscosity at non physiological condition (Y1) and Viscosity at physiological condition (Y2) served as variables. The optimized formulation is characterized by EE, particle size, Fourier transform infrared spectroscopy (FTIR), Xray diffraction (XRD), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), in vitro drug release study, antibacterial efficacy and antibiofilm activity. The percentage of EE is 89%, the size is 56.25 nm, and the in vitro drug release is 92%. Mfx-GNPs loaded in-situ gel are fully prepared and can effectively release drugs in a sustained mode.