Isolation And Identification Of Chemical Constituents From Herbal Syrup In Treating Fibroid

Abstract

Purpose:

 Fibroid is one of the reasons for infertility as well as heavy bleeding in women. The main source of fibroid growth is estrogen. Down-regulation of oestrogen helps to control fibroid growth. Various conventional medicines are prescribed to treat this fibroid. The present study aims to identify components from the syrup by combining two or three ingredients mixed together and analysing the components for their therapeutic activity in treating fibroid both in vitro and insilico. The syrup we prepared from Ficus carica bark powder, Prosopis juliflora bark powder, and Eujenia jambolana bark powder samples was equally mixed with methanol

Methods and Material: The following studies were performed, which are as follows: DPPH Assay, FTIR, GC-MS, and a computational docking study were used to Antioxidant activity studied using DPPH assay since free radical elevate the level of oesterogen, identify the components, and also investigate the efficacy of the components present in the syrup in controlling oestrogen regulation respectively.

Results:  DPPH assay showed the herbal extract's maximum antioxidant activity at a concentration of 80 g/ml.  The existence of O-H, C-C, C=O, NH3+ in amino acids, OH in carboxylic acids, C-N, C-OH in amino acids, C-O, and CH3 functional groups was validate by FT-IR analysis. Dodecanoic acid, Undecane, 1-Tetradecanol, Tetradecanoic acid, (+)-Cycloisolongifol-5-ol, Tridecanoic acid etc, compounds were identified using GC-MS analysis.

Conclusions: Based on the docking scores and hydrogen bond interactions, identified compounds bound well to the selected target proteins(aromatase enzyme, acetylcholine esterase, Beta Catenin, and progesterone receptor)  which help to control oestrogen regulation