Free Radical Scavenging And Antioxidant Enhancing Ability Of Moringa Oleifera Leaf Extract Against Gamma Radiation-Induced Enzyme Toxicity In The Liver Of Pangasius Sutchi Antioxidant Activity Of Moringa Oleifera Leaf Extract

Abstract

Radiotherapy is the most common treatment for cancer patients, where ionizing radiation kills the tumor and healthy non-cancerous cells surrounding them. A synthetic drug, amifostine is commonly utilized to prevent the major drawback of radiotherapy. This drug may also be toxic to the individual being treated. In this aspect, Moringa oleifera leaf – 30% ethanol extract (MOLE) shows abundant antioxidant-rich polyphenols, neutralizing free radicals from radiation penetration into living cells' water molecules. The free radical scavenging ability of MOLE in the liver of Pangasius sutchi can be quantified by antioxidant enzyme assays such as superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH), which are endogenous enzymes of our body and are involved in neutralizing free radicals. The lipid peroxidation (LPO) assay also quantifies the peroxidation level of fatty acids arising from lipoxygenases or free radical pathways. The default radioprotective effect of amifostine was high among the comparative compounds. This result was followed by MOLE and flavonoids such as kaempferol and quercetin. Thus, the study forms a base for the radioprotection activity of MOLE in replacing the chemical drug amifostine.