Physiological, In-vitro, and Analytical Studies of Cellular Oxidative Stress for Evaluation of Antioxidant Pharmacological Effects of Natural Polysaccharide in Fast Dissolving Tablet Formulations.

Abstract

Introduction: Inflammation represents a significant health challenge, necessitating effective therapeutic interventions. Glycyrrhiza glabra mucilage, a Natural Polysaccharide, has demonstrated promising anti-inflammatory properties. This study aims to evaluate the anti-inflammatory efficacy of Glycyrrhiza glabra mucilage through in vitro tests and formulate it into fast-dissolving tablets (FDTs) to enhance its pharmacological efficacy, particularly within physiological responses to inflammation.

Materials and Methods: Mucilage from Glycyrrhiza glabra was tested for anti-inflammatory effects using protein denaturation and membrane stabilization assays. Fast-dissolving tablets were also formulated with mucilage, sodium starch glycolate, and microcrystalline cellulose, optimized for wetting, disintegration, and drug release.

Results: The Protein Denaturation Inhibition Assay indicated that Glycyrrhiza glabra mucilage inhibited protein denaturation by 78.5% at the highest concentration. The Membrane Stabilization Assay demonstrated a 65.2% reduction in hemolysis, confirming its membrane-stabilizing properties, which are crucial for physiological integrity during inflammatory responses. The optimized FDT formulation achieved a predicted wetting time of 30.25 seconds, cumulative drug release of 95.43%, and disintegration time of 65.78 seconds, with observed results closely correlating with predictions.

Conclusion: This study successfully validated the anti-inflammatory properties of Glycyrrhiza glabra mucilage through in vitro assays, highlighting its physiological relevance in inflammation management. The development of effective fast dissolving tablets indicates its potential for therapeutic applications in managing inflammatory conditions. Further in vivo studies are warranted to confirm clinical benefits and elucidate the underlying physiological mechanisms.