Development and Characterization of Gamma-Oryzanol Loaded Folic Acid Conjugated Multi Walled Carbon Nanotubes

Abstract

Ancient cultures placed a great deal of importance on plants in their folklore. Plants have been used for more than 5000 years as remedies in addition to food and spices. It is estimated that between 70 and 95 percent of people in poor nations still consume. These days, plants containing important compounds that have therapeutic or helpful effects in the treatment and prevention of a wide range of human and animal disorders are referred to as medical herbs. Herbal goods have been used in addition to conventional medications as supplementary therapies. MWCNTs were functionalized one after the other to create γ-OZ/FA-MWCNTs. In phosphate buffer solutions (PBS) with a pH of 7.4, γ-OZ was physically loaded onto virgin MWCNTs and FA-MWCNTs. The lung epithelial cancer cell line "A-549" was used to evaluate the entrapment efficiency, in vitro release, and ex vivo tests. In both the pristine and FA-MWCNT formulations, γ-OZ was successfully incorporated, with the FA-MWCNTS formulation demonstrating the greatest γ-OZ trapping at 86.5 1.5%. The in vitro release of γ-OZ from γ-OZ/FA-MWCNTs at 7.4 (PBS) was investigated in the study, revealing a faster initial release followed by a sustained release. Additionally, γ-OZ/FA-MWCNTs was found to have a cytotoxic impact when used on the "A-549" lung epithelial cancer cell line.