TLC-Bioautography Profiling of Pippali Extract for Antidiabetic and Antioxidant Effect

Abstract

Pippali, also known as Piper Longum, has potential therapeutic uses, including antidiabetic and free-radical scavenging. The constituents responsible for Pippali's antidiabetic and antioxidantproperties were investigated. The in vitro inhibition of α-amylase and α-glucosidase enzymes and evaluated antioxidant activity through DPPH scavenging activity were assessed. TLC-bioautographic-MS analysis identified specific compounds accountable for Pippali's antidiabetic and antioxidant activities. The study found a significant inhibitory effect on α-amylase and α-glucosidase enzymes, indicating its potential in regulating carbohydrate absorption and digestion. Piperine, a compound present in Pippali, exhibited prominent antioxidant properties and α-amylase inhibition, suggesting its crucial role in Pippali's antidiabetic and antioxidant effects. These findings offer valuable insights into Pippali's potential therapeutic applications in managing diabetes and associated complications.