Effect Of Ultrasound-Assisted Extraction On Efficiency, Antioxidant Activity, And Tocopherol Content Of Passiflora Edulis Seed Oil

Abstract

Introduction: Context: Passiflora edulis is tropical fruits that contain 70% of seeds. The juice industry categorizes the seed as a waste. Even though the seeds contain oil which has great potential in the pharmaceutical industry.

Objectives: to optimize the efficiency of Ultrasonic Assisted Extraction (UAE) thought antioxidant activity and tocopherol contents. 

Methods: :  The extraction of Ultrasonic-Assisted Extraction with various parameters in temperatures (50^oC and 60^oC) and time (5, 10, 15, 20, 30 minutes). Antioxidant assay was used 1,1 Diphenyl 2-picrylhydrazyl radical scavenging and tocopherol content with TLC-Densitometry.

Results: The results of the study showed that extraction with UAE at temperatures of 50^oC and 60^oC for 30 minutes showed very good results with higher oil yields and antioxidant potential IC₅₀ of 14.996 and 14.865 ug/mL, while tocopherol levels were 32.302 and 33.097 ug/mL, respectively.

Conclusions: The results of the study showed that extraction with UAE at temperatures of 50^oC and 60^oC for 30 minutes showed very good results with higher oil yields and antioxidant potential by reducing DPPH IC₅₀ of 14.996 and 14.865 ug/mL, antioxidant with FRAP method IC₅₀ 50.592 and 47.076ug/mL, while tocopherol levels were 32.302 and 33.097 ug/mL, respectively