Effect of Combination of Hydroalcoholic Seed Extract of Areca Catechu With Gliclazide in Alloxan-Induced Diabetic Nephropathy in Wistar Rats

Abstract

Background and Objective: To study the renoprotective effect of hydroalcoholic seed extract of Areca catechu (AN) in conjunction with gliclazide in alloxan-induced diabetic nephropathy in male Albino wistar rats. Methods: To induce diabetic nephropathy, a single intraperitoneal injection of 150 mg/kg of alloxan monohydrate was given. Hydroalcoholic seed extract of A. catechu (AN) was administered at three doses i.e., 250mg/kg, p.o., 500mg/kg, p.o., and 1000mg/kg, p.o., and gliclazide (25mg/kg, p.o.) for a period of 28 days as an individual treatment as well as in combination. To evaluate the renal functions, various biochemical tests were carried out, including albumin, serum creatinine, BUN, kidney weight/body weight (%) ratio, and serum glucose. Total brain protein and reduced glutathione were estimated to measure oxidative stress. Lipid profile was evaluated by measuring serum cholesterol, triglycerides, and high-density lipoprotein (HDL). Results: Administration of AN and gliclazide individually as well as in combination significantly attenuated the serum glucose, BUN, serum creatinine, albumin levels, and oxidative stress. In the treated groups, serum lipids such as triglycerides and total cholesterol decreased while HDL increased. Conclusion: The outcomes of our investigation indicate that the glucose-lowering, lipid-lowering, and anti-oxidative properties of the polyphenols found in areca nuts may be responsible for the renoprotective effect of AN both when taken alone and in conjunction with gliclazide.