Studying the Effect of Amputation-Induced Oxidative Stress on Tadpole Tail Regeneration in Clinotarsus curtipes

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M. Sithi jameela , S. Ramesh Kumar

Abstract

Background and Aim: The role of oxidative stress, particularly reactive oxygen species (ROS), in tissue regeneration is increasingly recognized as crucial. This study aimed to investigate the role of ROS in the regeneration of tadpole tails in Clinotarsus curtipes following amputation, focusing on lipid oxidation, superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) activity.


Material and Methods: This prospective observational study was conducted in a controlled laboratory setting. The study population consisted of tadpoles subjected to tail amputation, and tissue samples were collected at regular intervals. Key biochemical markers, including lipid oxidation, SOD activity, catalase activity, and GSH levels, were measured to assess oxidative stress and antioxidant response.


Results: Peak antioxidant activity was observed at 15 days post-amputation. Hydrogen peroxide was identified as a significant contributor to the regeneration process. ROS levels were elevated early in the regeneration process, and a decline in lipid oxidation and increase in antioxidant enzyme activity was noted as regeneration progressed.


Conclusion: The findings suggest that ROS, particularly hydrogen peroxide, play a pivotal role in the regeneration of tadpole tails. Understanding the interplay between oxidative stress and tissue regeneration could have implications for developing therapeutic strategies for wound healing and regenerative medicine.


 

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