Spectral characterisation and assessment of triterpenoids isolated from leaf extract of Glochidion talakonense

Abstract

Glochidion talakonense belongs to the family Phyllanthaceae, is a tree, up to 7m high, that grows wild throughout Talakona, Seshachalam biosphere reserve in Chittoor district of Andhra Pradesh, India. In traditional medicine, this species has been applied to use for a various biological activity in traditional medicine. However, there is no information about chemical composition and bioactivity of this species. This study focuses on the isolation and identification of compounds from Glochidion talakonense. The methanol extract of the Glochidion talakonense leaf was separated by liquid-liquid extraction, followed by different chromatographic techniques to purify the chemical composition. The result showed that five terpenoids including lupeol (1), Glochidiol (2), Lupa-1,20(29) diene-3-one (3), Lup-20(29)-ene-3β,16β-diol (4), Lup-20(29) ene-1,3-dione (5) were isolated from the methanol extract. The chemical structures of these terpenoids were elucidated based on IR, NMR and MS spectral analysis, and by comparison with previous references. These compounds have not been previously isolated from Glochidion talakonense.