Molecular Study Of Insulin-Like Growth Factors-1 Gene In Patients With Various Stages Of Chronic Kidney Disease

Abstract

Introduction: Genetic research worldwide has been carried out to discover the genes that influence the prevalence of chronic kidney disease (CKD) in individuals. Insulin-like growth factor 1 (IGF-1) is one of these genes involved in CKD emergence.

Objectives: Given the likely role of IGF-1 in advanced stages of CKD, we sought to examine its expression levels and their impact on renal function in patients with varying stages of renal disease.

 Patients and Methods: This case-control study comprises 55 patients diagnosed clinically and serologically with CKD, their age varies from (18 to 75 years) and 20 individuals as control. Blood samples were collected to extract RNA, then converted to cDNA, and the gene expression was detected by amplifying using a real-time polymerase chain reaction technique. The levels of mRNA IGF-1 genes were normalized by amplifying the endogenous control gene B-actin. The Excel and SPSS were among the statistical programs and software utilized for quantitative polymerase chain reactions.

Results: IGF-1gene has significant overexpression in CKD patients, also the expression in males increases 2-fold in females. IGF-1 gene folding elevated gradually with the progression of stages of CKD. Moreover, the highest value of expression was registered in the advanced stages of disease.  The folding was assessed in different age groups and showed elevated in the early ages and decreased in elderly patients.

Conclusion: We concluded that IGF-1 overexpression at the level of a biomarker could potentially assist in identifying those who are most at risk of developing CKD and help prevent and treat them early.