Comparative evaluation of In-vitro antioxidant and antimicrobial potential of different extracts of bark and leaves of Morus alba
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Abstract
This study aims to identify phytochemicals, assess the antioxidant capacity and antimicrobial activity of plant such as Morus alba by utilising a bark and leaves. A recognised testing approach was used to determine the qualitative analysis of several phytochemical components. An 50% ethanolic extract of Morus alba bark-leaf mixture was subjected to an in vitro antioxidant activity study using the 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay, superoxide radical scavenging assay, and reducing power determination. Phenols and flavonoids were found by phytochemical investigations. The petroleum ether, chloroform, ethyl acetate extract and 50% ethanolic extract of different parts like bark and leaves of Morus alba were tested for activity against Streptococcus mutans, Salmonella bongori and Escherichia coli pathogens by using agar well diffusion assays.
The blend of morus alba bark-leaves had an IC50 value of 63.061µg/ml, for the DPPH method, which was comparable to ascorbic acid (IC50=57.594 µg/ml). The extract exhibited dose-dependent free radical scavenging capacity in the models that were studied. In the models that were examined, the extract showed dose-dependent free radical scavenging ability. In the reducing power determination, the IC50 value was found to be 88.487 µg/ml, which was comparable to ascorbic acid's IC50 value of 77.762 µg/ml. The maximum antimicrobial activity was shown by 50% Ethanolic extract fraction of bark and leaves. The zone of inhibition of bark extract 19±0.15, mm, 21±0.72 mm, 22±0.51 mm was observed against S. mutans, S. bongori, Escherichia coli respectively. In case of leaves extract, zone of inhibition of 11±0.24 mm, 24±0.36 mm, 18±0.18 mm was observed against S. mutans, S. bongori, Escherichia coli respectively. This research describes the phytochemical profile, antioxidant activity and antimicrobial activity of morus alba, which will be used for therapeutic applications.