Study of serum matrix metalloproteinase-1 [MMP-1] in Knee osteoarthritic postmenopausal women

Abstract

Objective

To determine the association of serum MMP-1 between postmenopausal females with knee osteoarthritis (Case) and postmenopausal females without knee osteoarthritis (Control).

Design

Case-control study

Method

The study involved 200 female subjects between the ages of 45-70 years. Out of these, 100 were healthy female postmenopausal controls, while the other 100 were postmenopausal female patients suffering from knee osteoarthritis. The study protocol was approved by the Institutional Ethical Committee of UPUMS, Saifai, Etawah. Serum MMP-1 levels were measured in all samples using an ELISA analyzer, following the manufacturer's instructions on a commercial kit. The results are expressed as arithmetic means with standard deviation, and a p-value of less than 0.05 is considered statistically significant. Paired sample t-test was used to analyze the data.

Results

It was observed that the level of Serum MMP-1 was significantly higher (p=0.0001) in cases (533.92±178.51) as compared to controls (42.76±29.19). This finding suggests that MMP-1 levels can be considered a reliable biochemical marker for the diagnosis of osteoarthritis (OA).

Conclusion

Postmenopausal women with knee OA show a significant association with serum MMP-1, making it a useful diagnostic marker.