Simultaneous Quantification of Luteolin and Curcumin in Herbal Supplements Using RP-HPLC for Addressing Nutritional Deficiencies.

Abstract

Background: Medicinal plants are rich sources of bioactive compounds, primarily secondary metabolites, contributing to their therapeutic and nutritional properties. Moringa oleifera and Curcuma longa are widely recognised for their high nutritional value, with luteolin (a flavonoid) and curcumin (a polyphenol) as their primary active constituents. These compounds possess antioxidant and anti-inflammatory properties, effectively addressing nutritional deficiencies.

Objective: This study aimed to develop and validate a Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for simultaneously estimating luteolin and curcumin in herbal formulations used for nutritional supplementation.

Methods: Herbal tablet and granule formulations containing Moringa oleifera and Curcuma longa extracts were prepared. The RP-HPLC method was optimized and validated according to ICH guidelines, evaluating parameters such as precision, accuracy, linearity, and robustness. The formulations' physicochemical parameters were also assessed.

Results: The developed RP-HPLC method demonstrated excellent precision, accuracy, and linearity for simultaneously estimating luteolin and curcumin. The herbal formulations exhibited acceptable physicochemical properties, and the presence of luteolin and curcumin in significant quantities was confirmed.

Conclusion: The validated RP-HPLC method provides a reliable and robust approach for the quality control and standardization of herbal formulations containing luteolin and curcumin. These formulations show potential as effective nutritional supplements, suitable for all age groups, supporting their use in addressing nutritional deficiencies.