Immunomodulatory Potential Of Isolated Compound Of Vigna Unguiculata Leaves

Abstract

Aim: The main aim of the present study is to investigate the immunomodulatory activity of phytochemical compound isolated and purified from methanolic extract of Vigna unguiculata leaves by different animal models.

Methods: The isolated compound of Vigna unguiculata leaves extract was compromised to acute toxicity according to OECD guidelines. Healthy albino Wistar rats of either sex was used for this study. The effect of the component on DTH response using SRBC was investigated by the footpad thickness, humoral antibody response to SRBC, carbon clearance assay or phagocytosis and cyclophosphamide induced neutropenia was investigated.

Result: The isolated compound of Vigna unguiculata leaves demonstrated the increase in paw thickness compared to control and standard group. The compound caffeic acid was having stimulatory effect on humoral immunity; this judgement was on the basis of effect on increment in HAT value. The increase in phagocytic index reflects the enhancement of the phagocytic function. The compound showed increase in total leukocyte count and differential leukocyte count.

Conclusion: This study revealed that the isolated compound, caffeic acid possessed immunomodulatory effect by stimulating cellular immunity, humoral immunity, phagocytosis and neutropenia.