Design And Synthesis Of Nanoparticle-Encapsulated Drug Delivery Systems For Targeted Cancer Therapy: Exploring The Role Of Green Chemistry And AI-Driven Molecular Modelling

Abstract

In order to determine the localisation in the breast cancer model system, the newly created polymeric Poly-ɛ-caprolactone (PCL)/pluronic F108 nanoparticles loaded with an anticancer medication and near infrared (NIR) dye are prepared, characterised, and applied in this study. The nanoprecipitation approach was used to create poly-ʛ-caprolactone (PCL)/pluronic F108-based nanoparticles loaded with docetaxel and NIR dye,"1,1′-dioctadecyl-3,3,3′,3′-tetra methyl indotri carbocyanine iodide (DiR)". Numerous physicochemical techniques, such as size distribution, zeta potential, surface morphology, loading capacity, drug entrapment efficiency, differential scanning calorimetry, in vitro release, and kinetics, were then used to characterise this formulation. The anticancer effectiveness of the nanoparticles was assessed using in vitro cell proliferation tests of human breast cancer BT-474 cells. The contribution of pluronic F108 to the uptake of the nanoparticles by cells was also assessed. Noninvasive infrared dye imaging was used to investigate the localisation of drug- and NIR dye-loaded nanoparticles at the tumour location. The optimised nanoparticle formulation had a zeta potential of -7.37 mV ± 4.93 mV, a particle size of 100-300 nm, and a polydispersity index of 0.156. Diffusion-mediated drug release was suggested by the nanoparticle system's drug release, which followed Higuchi kinetics. According to DSC measurements, the medication in the nanoparticle formulation is either in an amorphous or disordered crystalline state. The potential of PCL/F108 nanoparticles as an enhanced targeted drug delivery method is indicated by their higher accumulation in vivo.