Pharmaceutical Analysis Of Felodipine Via Various Analytical Methods

Abstract

The morphological and physical characterization of felodipine forms (microcrystalline and macrocrystalline) using Fourier transform infrared spectroscopy, differential scanning calorimetry, and optical microscopy was done first, and the pharmaco-technical properties ofthe felodipine forms were also determined. This paper describes the analytical method suitable for validation of Felodipine by High Performance Liquid Chromatography (HPLC) method, which used Water 2695 with PDA detector. The column used in m) with a flow HPLC was C18 (4.6 x 150mm, 5 rateof1.0 ml/min).After being degassed ina sonicator for approximately ten minutes, the mobile phase, which is composed of ACN: Water (85:15% v/v), had an injection volume of 10µl and an ultraviolet detection wavelength of 362 nm. The procedure was carried out and verified in compliance with ICH norms. The procedure is quick, precise, accurate, dependable, and repeatable, according to validation. The calibration curve plots showed a linear relationship between concentrations 5 and 35 μg/mL (R2=0.999) . The limit of quantification (LOQ)was 0.62 µg/ml, while the limit of detection (LOD) was 0.21 µg/ml. The accuracy, repeatability, and consistency of the suggested approach were determined. It was successfully used to analyze the drug in a commercial formulation and might be applied to routinely analyze formulations that contain felodipine.