Siddha Science: Unlocking the Herbal LCZ Mixture's Anti-Inflammatory Secrets for Modern Healing

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Saravanasingh Karan Chand Mohan Singh, Suresh Kumarasamy, Lakshmanakumar Venkatachalam, Ethel Shiny.S, Suguna Mani, Anbarasan Balasubramanian, Gnanavel P, Devaki R, V. Gowri, P. Bama

Abstract

Introduction: Leucas aspera, Coccinia grandis, and Zingiber officinale (LCZ) are commonly used in Asia's Ayurvedic, Siddha, and Unani conventional medical practices. Different portions of these plant materials have historically been employed in traditional medicine for various functions, like anti-diabetic, anti-bacterial, anti-inflammatory, etc. Siddha-based formulation mixtures for medicinal uses are considered an efficient alternative to synthetic substances because of their side effects. Aim and Objective: The research aims to find an in-vitro anti-inflammatory action of the herbal-based Siddha formulation mixture of Coccinia grandis, Leucas aspera, and Zingiber officiale. Methods: The anti-inflammatory activity of the herbal-based Siddha formulation mixture of Coccinia grandis, Leucas aspera, and Zingiber officinale was evaluated using in vitro-based assays: protease inhibition assay, protein denaturation inhibition assay, and phytochemical analysis. This study mainly focuses on finding the most potent plant extracts and their effectiveness. All data are presented as the mean ± standard deviation for all in vitro assays tested, and each analysis was done in triplicate. One-way analysis of variance (ANOVA) was performed using MINITAB 15 software and Pearson’s correlation coefficient (r) with the level of significance (p ≤ 0.05) (2-tailed) Results: The LCZ sample is analyzed for the protease inhibition assay, and the higher (500µg) concentration of the specimen mean value was identified as 37.4864%. In the albumin denaturation assay, the higher (500µg) concentration of the specimen mean value was 80.9151%. The LCZ sample's IC50 value was 68.56 µg/ml for the protease inhibitory assay. For the primary albumin denaturation assay, it was 42.66 µg/ml. Conclusion: The outcomes of our study propose that all portions of plants exhibit potent anti-inflammatory action. The existence of most general phytochemicals in LCZ extract may account for their medicinal impacts.


 

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