To Study Phytochemical Investigation and Antibacterial Study of Azadirachta Indica against Enterococcus Faecalis
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Abstract
Background and Objectives: This study looked into the phytochemical components and antibacterial activity of A. indica extracts against E. faecalis, a varied group of facultative anaerobic, gram-positive bacteria that could be used to create new therapeutic approaches.
Material and Methods: The phytochemical makeup and antibacterial activity of A. indica against Enterococcus faecalis (E. faecalis) were looked at in this work. The active ingredients from A. indica leaves were taken out using both water and ethanol as solvents. We used the disc diffusion method to test how well both A. indica extracts killed E. faecalis bacteria at different amounts. The zones of inhibition showed how sensitive the bacteria were.
Results: According to this study, both samples had phytochemicals in them, such as tannins and phenolics. In addition, the water-based extract only had alkaloids, flavonoids, and saponins. Also, steroids were only found in the ethanolic extract. The ethanolic and aqueous extracts were both successful against E. faecalis, with 20 mg/mL being the lowest concentration needed to stop growth. As the concentration of the extract went up, the inhibition zones got bigger. The ethanolic extract gave the biggest reading, 9.6 mm. This study shows that both ethanolic and water-based extracts of A. indica are very good at killing germs because they stop E. faecalis from growing.
Conclusion: According to these results, neem could be used to make new medicines, and they also show that it has been used traditionally as an antibacterial agent.