The ability of Escherichia coli isolated from different clinical cases to form biofilm and the detection of CsgA and YjaA genes
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Abstract
One hundred forty five samples were collected from various clinical sources at several hospitals in Baghdad city. After collection, the cultures were diagnosed, microscopic examinations and biochemical examinations. By Vitek-2 system, (50 isolates) were obtained from Escherichia coli with percentage 50(34%) distributed as follows: urinary tract infection (72%), wounds (14%), respiratory infection (2%), sputum (10%), vaginal swab (2%). After that detect of biofilm, antibiotic screening and molecular detection. The ability of E. coli to form a biofilm is one of the utmost important virulence factors and plays a role in causing infection and increasing antibiotic resistance to E. coli by using the microtiter plate method. The results indicated that (100%) of bacterial isolates were produced biofilm in different percentage, where the percentage of biofilm was weak in 11 isolates rate (91.6%), and in one isolate was medium rate (8.3%). The highest resistance to antibiotics (Cefazidime, Colistin and Ceftriaxone) with a percentage of 100% for 12 isolates. Molecular detection was chosen for the availability of the two genes responsible for the formation of biofilms (CsgA), (YjaA) for 12 isolates in E. coli, according to their ability to produce a biofilm, and thus the results appearedĀ that (100%) of 12 isolates carrying (CsgA) and (YjaA) genes.