Molecular Comparison Between Some Tissue-Planted Date Palm Taxa And Natural-Planted Date Palm Taxa In Iraq Using Rapd-Pcr

Abstract

This study uses the RAPD-PCR technique to carry out a molecular analysis using 12 different varieties of domestic and foreign date palm cultivars to determine the genetic similarity and relationship between some tissue propagating and non-tissue propagated date palm cultivars. 870 bundles, including 113 distinct bundles, were created by these primers. The double bundles had sizes ranging from 100 to 2000 base pairs. With a percentage of polymorphism of 20.87%. The OPC-02 primer had the highest values for firmness and reach (91) and (19) bundles with polymorphism. Its primer efficiency was 10.45%, and its discriminatory ability was 16.81. While the primer N17 provided the value for the primer efficiency (1.14) but the primer FO6 and M19 provided the least prefixes in the number of polymorphic bunds and the percentage of polymorphism amounting to (0), and the primer N17 provided the fewest output bunds (10). The two cultivars Umm al-Dahn (locally) and Majhoul (International tissue) are the most closely related cultivars from a genetic point of view with a similarity ratio of (0.714), while Barhi (local) and Umm al-Dahn (tissue) are more distinct and have a similarity ratio of (0.040). The percentage of genetic similarity between the studied date palm cultivars ranged from 0.040 to 0.714, Except for the two varieties Barhi (locally) and Sakkai (International tissue), the cluster analysis revealed the convergence of the free and (international tissue) varieties in one subgroup and the separation of each tissue variety into a separate group.