INVESTIGATION OF THE ANTIBACTERIAL ACTIVITY, ANTIBIOFILM PROPERTIES AND PHYTOCHEMICAL COMPOSITION OF POLY-HERBAL EXTRACTS OF PERESKIA ACULEATA, TRACHYSPERMUM AND SPHAGNETICOLA TRILOBATA

Abstract

The global problem of increasing resistance to antibiotics has prompted researchers to seek out new antimicrobial agents derived from natural compounds. The objective of the present investigation was to assess the antibacterial efficacy of commonly used but unexplored polyherbal formulations in the context of wound recovery treatment. A total of 3 kinds of plants were combined in varied quantities to generate 11 polyherbal concoctions. Various solvents were used to extract the constituents of 11 polyherbal concoctions. The extraction process involved the use of cold maceration after collecting and grinding the plant materials. The antibacterial activities of the polyherbal extracts were tested using the technique of diffusion on agar wells at a dosage of 30 mg/ml. The MIC (minimum inhibitory concentration) was estimated using the sequential dilution method. The polyherbal concoction (G) had the greatest efficacy against S. aureus (37±1.0 mm), E. coli (29±1.5mm),  and B. subtilis  (35±0.40 mm). The lowest inhibitory concentration was also assessed using a ratio of 1:1:2:1, consisting of Pereskia aculeata, Sphagneticola trilobata leaves, and  Trachyspermum ammi seeds and leaves. The minimum inhibitory concentration (MIC) values against E. coli, S. aureus  and B. subtilis were determined to be 50±0.12 mg/mL, 70±0.17 mg/mL, and 30±0.20 mg/mL, correspondingly. The antibacterial efficacy of polyherbal recipe G is enhanced, surpassing that of other examined recipes. This recipe warrants further scientific scrutiny for additional validation.