Green Synthesis Of Selenium And Zinc Oxide Nanoparticles Using Andrographis Paniculata And Its Anti-Inflammatory Effect On Hepg2 Cell Line

Abstract

The present study focuses on the green synthesis of selenium and zinc oxide nanoparticles using Andrographis paniculata, a medicinal plant known for its rich phytochemical content. The plant extract served as a natural reducing and stabilizing agent, enabling an eco-friendly synthesis approach. Characterization of the synthesized nanoparticles confirmed their stability and the presence of functional groups responsible for bioreduction, as revealed by FTIR analysis. The cytotoxicity of the nanoparticles was evaluated using the MTT assay on HepG2 cells, revealing a dose-dependent response. Notably, lower concentrations (1–10 µg/ml) exhibited reduced toxicity, indicating their potential biocompatibility. Furthermore, the anti-inflammatory activity of the nanoparticles was assessed using the albumin denaturation assay, demonstrating significant inhibition, especially at lower doses. These findings suggest that selenium and zinc oxide nanoparticles synthesized via Andrographis paniculata possess promising anti-inflammatory properties and may serve as potential candidates for therapeutic applications with minimal cytotoxic effects.