Molecular Detection Using A Particular Primer For The Isolation Of Pseudomonas Aeruginosa From A Persistent Bacterial Co-Infection In A COVID-19 Patient.

Abstract

Bacterial co-infections increase the severity of respiratory viral infections and are frequent causes of mortality in COVID-19 infected subjects. We aimed to investigate the antimicrobial resistance patterns and molecular typing of Pseudomonas aeruginosa isolates among Coronavirus disease-19 patients. According to some, one of the most nosocomial bacteria is Pseudomonas Aeruginosal. Between October 2021 and June 2022, 100 sputum samples were collected for this study from several Wasit hospitals. twenty seven (27%) isolates were identified as Pseudomonas aeruginosa by routine biochemical tests, vitek 2 system ; In attempting to the identification of P. aeruginosa strains at the DNA level, Polymerase chain reaction (PCR) is used based on specific primer (27F/1492R) for (16S rRNA) gene. The results showed that PCR has found to be rapid and more sensitive and specific in identification of P. aeruginosa. During the COVID-19 period, especially at the beginning of the pandemic, an inappropriate use of broad-spectrum antibiotic treatments has been frequently described, mainly due to prolonged hospitalization, and the use of immune-suppressive treatments as steroids. P. aeruginosa strains were resistant to imipenem followed by meropenem , ciprofloxacin and levofloxacin .