The Importance Of Epigenome And Microbiome In The Regulation Of Endocrine-Mediated Inflammation In Diet-Induced Obesity

Abstract

Microbiomes, also known as epigenomes, may have a role in metabolic health and the inflammation associated with diet-induced obesity (DIO), according to recent research. Examining the role of endocannabinoids in regulating inflammation in a mouse model, this work delves into the complex relationship between DIO, the epigenomic landscape, and the microbiome makeup of the digestive tract. To study the effects of a high-fat diet (HFD), researchers first made C57BL/6J mice overweight and then monitored their weight. Discovering alterations in metabolic parameters or inflammatory indicators, or both, was the aim. Researchers discovered changes in DNA methylation patterns linked to obesity by integrating genomic sequencing with epigenetic profiling. The most notable changes were seen in the genes that control inflammation and lipid metabolism. At the same time, scientists analyzed the gut microbiota using 16S rRNA sequencing and found that the HFD was associated with major changes in the variety and composition of microbes. The rise in inflammatory-promoting microbial taxa, linked to increased levels of endogenous THC, was the most striking finding. Researchers assessed the impact on inflammatory responses and metabolic repercussions after pharmaceutically modifying endocannabinoid signaling to identify the pathways. The results show that inflammation in DIO is influenced by both the microbiota and the epigenome. Endocannabinoid signaling is subsequently affected by this. This study demonstrates the complicated interaction between nutrition, microbiome, and epigenetic pathways in metabolic health and the possibility of addressing inflammation linked to obesity by targeting these pathways as a treatment strategy; however, further investigation is required to ascertain the precise mechanisms involved and their consequences for the prevention and treatment of obesity.