Production and Optimization of Biosyntheses Gold nanoparticle(AuNPs) from Bacillus subtilis

Abstract

   Gold nanoparticle (AuNPs) have many advantages and awide rang of application in various fields,for that, thereis agrowing interest for the syntheses of AuNPs using an economical ,safe and less complex,This study demonstrated that the extracellular of biosynthesis gold nanoparticle (AuNPs)using Bacillus subtilis, characterized using Uv-Vis spectroscopy,and studied optimization  condition of culture  broth using OVAT method. strain of  Bacillus subtilis was isolated from local soil sample to enhance the production of gold nanoparticle by the bacteria. The bacteria extracellular extract production in  Brian Heart Broth. the whole optime condition  was conducted at 150 rpm stirring speed. The highest amount of gold nanoparticle synthesis was demonstrated by the isolated Bacillus subtilis at 37°C, 96 h of incubation, pH 7, Starch carbon source, and Beef extract nitrogen source. Optimized medium is give double resulte for AuNPs producion.