Enhancement And Evaluation Of Phenolic And Flavonoid Content In Plant Cell Suspension Cultures Of Passiflora Foetida Linn Using Abiotic Elicitor Enhancement and Evaluation of Phenolic and Flavonoid Content

Abstract

By using plant tissue culture methods, many active phytoconstituents of medicinal plants are studied.Plant tissue culture proves as an efficientplatform for such studies. Undifferentiated masses of cells through in-vitro callus culture development under aseptic conditions and in specific media are generated by using medicinal plants. The undifferentiated cells produce and secrete vitalactive pharmaceuticals when they are cultured ina suspension medium. Passiflorafoetida Linn.(a medicinally active plant) contains different active phytoconstituents which have high therapeutic value. In this investigation, callus and cell suspension cultures from leaf explants of Passiflorafoetida Linn.are optimized and the application of the abiotic elicitor (i.e. Salicylic acid at concentrations of 50 uM, 150 uM, and 250 uM) is evaluated.At 24 and 96 hours, cell suspension cultures are harvested (intracellular and extracellular) respectively.After that phenolic and flavonoid components are analysed quantitatively using standard spectrophotometric methods using standards (i.e.gallic acid and quercetin).The results indicate the enhancement in the phenolic and flavonoid contents after 24 hours of application is seen when Salicylic acid elicitation is done at 150 uM concentration. This finding proves that salicylic acid is very effective for enhancing the phytochemical production in Passiflorafoetida Linn. under aseptic tissue culture conditions.