PHYTOCHEMICAL PROFILING OF MOMORDICA CHARANTIA L., SEEDS USING GC-MS ANALYSIS

Abstract

This research focuses on the phytochemical profiling of Momordica charantia seeds using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Momordica charantia, also known as bitter melon, has been widely used in traditional medicine for its various biological properties, including anti-diabetic, anti-inflammatory, and antioxidant activities. The general objective of this study is to explore the ethnobotanical uses, biological properties, and chemical composition of Momordica charantia seeds. The study is conducted in two phases: Phase I involves the collection of Momordica charantia seeds, followed by extraction using aqueous and methanol solvents. Preliminary phytochemical tests are performed to identify bioactive compounds such as alkaloids, flavonoids, and saponins. In Phase II, the extracts are subjected to GC-MS analysis to identify and characterize the isolated compounds. The GC-MS technique will provide a detailed chemical profile of the seeds, offering insights into the specific compounds responsible for their medicinal properties. This study aims to contribute to a deeper understanding of the pharmacological potential of Momordica charantia seeds, supporting their traditional uses while uncovering new therapeutic applications.