Evaluation of DNA Protective Properties among Various Trigonella Species

Abstract

Background/aim: Trigonella strangulata Boiss., Trigonella filipes Boiss., and Trigonella uninata Banks & Sol. are three widely used species that grow well in the northern suburbs of Iraq and are highly used in oral consumption and folk medicine. In this study, the effect of the different extracts from Trigonella filipes, Trigonella strangulata, and Trigonella uncinata plants with pBR322 plasmid DNA tertiary structure was investigated.

Materials and methods: In this study, the effect of the different extracts from Trigonella filipes, Trigonella strangulata, and Trigonella uncinata plants with pBR322 plasmid DNA tertiary structure was investigated by their ability to modify the supercoiling of closed circular pBR322 plasmid DNA.

Results: According to this protocol, the activity of the plant extracts to prevent DNA damage was increased in the presence of hydrogen peroxide (H₂O₂) and UV light, which are factors that cause damage to DNA. We used three different solvents for plant extraction (Hexane Extraction, Ethanol Extraction, and Water Extraction), and we used the Soxhlet method for extraction. The DNA protective action of the water and ethanol extracts of Trigonella species was better than hexane extracts. It was found that hexane extracts of all 3 plant species showed lower activity in terms of DNA defensive activity potentials. It was observed that the DNA protective activity of the T. strangulata plant was high at all doses applied with water and ethanol extracts, and Form 1, 2, and 3 DNA bands of pBR322 plasmid DNA.

Conclusion: It was found that the water extract and ethanol extract of the T. uncinata plant showed a better level of DNA protective activity than the hexane extract. The pBR322 interaction results of these plants demonstrate that these plants have been able to cause moderately conformational changes in DNA.