Traditional knowledge application of Cordyceps Sinensis and trace elements analysis by XRD(x-ray diffraction technique) and Graphic furnace- Atomic absorption spectrometer (GF-AAS)

Abstract

Tibetans history records the first uses of yartsa gunbu in the 15^th century.Cordyceps is considered to be derived from the latin word cord (club), ceps (head), andsinensis (from china).Then fruiting body and attached mycelium of cordyceps have been used in Chinese culture on and in traditional Chinese medicine for centuries.Cordyceps is valued for its activity in restoring energy, promoting longevity, and improving quality of life. Not contrary of these activities, Bhutanese indigenous doctors are also using so commonly in their region. The presence of trace element (mineral elements)in the aqueous extract of C.sinensis will contribute to therapeutic application in medicinal practitioners.

Cordyceps Sinensis have wide range characteristics as enhancing body’s immune function, anti-aging, antidiabetic, anti-inflammatory, anti-fatigue, anticancer, antibacterial, antivirus, androgen function, hypolipidemic and hypoglycemic effects. The aim of this study was to investigate the presence of trace elements or mineral contents in the methanoic extract of C.Sinensis.The methanolic extract was analyzed through X-Ray diffraction technique and Atomic absorption Spectroscopy for identification of phytoelement or trace element in C.Sinensis. The current study investigated about phytoelement in C.Sinensis.AAS analysis of methanol extract of C.Sinensis showed six elemental composition in methanolic extract of C.Sinensis.In extract tested,the highest conbcentration for Ferum( Fe) while Cr level was the lowest.The decreased in heavy metal of extract were in order of Fe>Mn>Cr>Cu>Zn>V.In XRD,it gives better resolution for Phytoelements which cannot be detected by AAS.It shows the presence of Na,K,Ca,V,Si,Se and Ni which cannot be detected by AAS. The result shows the presence of numerous phytoelement or mineral elements in the methanolic extract of C.sinensis which have medical importance and provide justification for their pharmaceutical responses.