Molecular detection of vanA, vanB genes and identification of 16SrRNA gene in E. coli isolated clinically and from water samples in Baqubah city

Abstract

    This study included collecting 211 samples, including 140 clinical samples (urine, swab burns, swab wounds, urine of patients with renal failure) from Baquba Teaching Hospital and Baladrooz General Hospital for both genders and different ages, and 71 water samples from the Public Health Laboratory of the Food Department in Diyala Governorate to check for the presence of E. coli bacteria. 44 samples were isolated and diagnosed at a rate of 20.85% based on cultural, microscopic, biochemical and Vitek tests. All bacterial samples were tested for sensitivity to 11 antibiotics, and the highest resistance rate was to vancomycin (100%), amoxicillin and ampicillin (86.84%), cefotaxime (84.21%), and the highest sensitivity rate was to meropenem and imipenem (100%).  For the purpose of accurate diagnosis of isolates by molecular methods, E. coli bacteria were diagnosed using a specific primer for the 16S rRNA gene. The results showed that the occurrence rate of this gene was 100%. An evolutionary tree was built for the new isolates, in addition to registering (3) isolates in the National Center for Biotechnology Information (two clinical isolates and one aquatic isolate). As for molecular detection, the occurrence rate of the Van A gene was (36%) (n = 9 isolates) and the occurrence rate of the Van B gene was (16%) (n = 4 isolates).