Pravastatin Effect on soluble Fms-like tyrosine kinase-1 (sFlt-1), Tumor Necrosis Factor-α (TNF-α), Interleukin-10 (IL-10) Expression and Hepatocyte Cell Necrosis in The Liver Pregnant Rat Model of Preeclampsia

Abstract

Introduction: Pravastatin is a reductase inhibitor that is able to inhibit HMG-CoA activity. Influences Heme Oxygenase-1 (HMOX-1) in producing CO and activating eNOS, thereby balancing angiogenic factors and reducing inflammation. This study objectives to determine the effect of pravastatin on the expression of tyrosine-1 such as soluble FMS (sFlt-1), Tumor Necrosis Factor-α (TNF-α), Interleukin-10 (IL-10), and the amount of hepatocyte cell necrosis in a pregnant rat model. preeclampsia.

Method: True experimental design with post test only control group design used liver tissue of wistar strain pregnant rats (Rattus norvegicus) as a preeclampsia model by administering pravastatin in 3 different doses.

Results: The expression of sFlt-1, TNF-α, and IL-10 as well as the number of hepatocyte cell necrosis in the five observation groups had a p value <α. Dose correlation with sFlt-1 expression (p-value 0.008; r = -0.576), TNF-α expression (p-value 0.001; r = -0.669), IL-10 expression (p-value 0.002; r = 0.644), number of hepatocyte cell necrosis (p-value 0.006; r = -0.591). Correlation of sFlt-1 and TNF-α expression (p-value 0.027; r = 0.594), sFlt-1 and IL-10 expression (p-value 0.007; r = -0.686), sFlt-1 expression with hepatocyte cell necrosis (p -value 0.034; r = 0.537), TNF-α expression and hepatocyte cell necrosis (p-value 0.049; r = 0.413), IL-10 expression, hepatocyte cell necrosis (p-value 0.045; r = -0.520).

Conclusion: Pravastatin is able to reduce the expression of sFlt-1, TNF-α and the amount of hepatocyte cell necrosis, as well as increasing the expression of IL-10. Significant results were found at a dose of 8 mg.