Estimation of Antioxidant, Antimicrobial and Antifungal Activity of Nelumbo nucifera

Abstract

Background: This study's goal was to conduct preliminary phytochemical screening, physicochemical screening, determine the total quantity of flavonoids and phenols, and antioxidant activity (by DPPH, PRA) antimicrobial activity (by disk diffusion method, MIC) and antifungal activity (by Inoculum, MFC).

Material and methods: Physicochemical and phytochemical screening of Nelumbo nucifera, testing of TPC, TFC in extract. Antioxidant activity was determined using the DPPH method and power reducing assay. Antibacterial effect was observed determined utilizing the disk diffusion procedure and MIC, and Antifungal activity was determined using of inoculum method and MFC.

Results: TPC extract was determined at the level of 25.12 ± 3.58 mg/g, while TFC extract was determined at the level of 34.23 ± 4.84 mg/g. The DPPH activity test shows IC50 34.05342 ±, reducing power assay was determine at the level of 2.02 ± 0.05, the disk diffusion method shows zone of inhibition was 12.5mm and 12.6mm and MIC was shows in 100mg/ml and the inoculum shows zone of inhibition was 11mm and 10.7 mm, and MFC was shows in 100mg/ml.

Conclusion: It was assessed that Nelumbo nucifera's methanolic extract has an antioxidant, antimicrobial and antifungal characteristics.