Metoprolol succinate and Olmesartan medoxomil spiked in human plasma for simultaneous estimation of antihypertensive drugs using RP-HPLC

Abstract

Introduction: To create a quick, precise, and economical HPLC approach for the simultaneous quantification of metoprolol succinate and olmesartan medoxomil in a combination tablet formulation, a simultaneous equation technique has been devised.

Materials and Methods: The method uses acetonitrile as a solvent and is based on calculations that allow for simultaneous analysis of both medications. Olmesartan medoxomil and metoprolol succinate have absorption peaks in acetonitrile at 225 nm.
Results: Olmesartan medoxomil and metoprolol succinate showed linearity at concentrations of 5 and 25 μg/ml and 4 and 20 μg/ml, respectively. To find the medicine amounts, the simultaneous equations method was used. For metoprolol succinate, the average recovery time was 97.72 ± 2.009 hours, and for olmesartan medoxomil, it was 98.02 ± 1.28 hours..
Conclusion: This method makes it easy, accurate, and precise to find both and olmesartan medoxomil metoprolol succinate at the same time in pharmaceutical tablet format. Recovery studies and statistical evidence support the research's findings.

 KEYWORDS: Acetonitrile, metoprolol succinate, HPLC, olmesartan medoxomil, recovery, validation, method development.