Phylogenetic Analysis and Antioxidant Activity of the Endophytic Bacterial Isolates from Kalanchoe pinnata

Abstract

Endophytic bacteria are vital members of the plant microbiome, and their phylogenetic diversity has been extensively documented using next-generation sequencing. They are also essential for initiating different biological effects and regulating the synthesis of secondary metabolites with important therapeutic qualities. Plant tissues harbor endophytic bacteria that generate bioactive chemicals that enhance plant growth, increase resistance to infections, and promote plant tolerance to stress. The aromatic plant Kalanchoe pinnata possess unique chemical elements, including essential oils such as lipids, alkaloids, triterpenes, steroids, and flavonoids, which impart it with great therapeutic potential and medical significance. The present investigation separated endophytic bacteria from Kalanchoe pinnata. Three endophytic bacterial isolates, HMA1, HMA2, and HMA3, were isolated after surface sterilization. These isolates were characterized using biochemical parameters and 16S rRNA sequencing. Phylogenetic analysis and 16S rRNA sequencing data indicated that the bacterial isolates were members of the Bacillus genera. A more comprehensive understanding of the microbiome of this plant could highlight the importance of studying endophytes and their potential applications in agriculture, medicine, and environmental biotechnology