Anti-inflammatory Effects of Atorvastatin in Polymicrobial Sepsis

Abstract

Sepsis, a potentially fatal illness caused by infection, frequently leads to the failure of multiple organs, including the heart. The purpose of this study was to examine the possible mechanism of action of atorvastatin in improving cardiac function after sepsis. 35 Swiss albino male  mice, weighing 30-38 g and aged 8–12 weeks, were divided in to five groups at random seven mice in each group, The control group continued to consume their regular food until the sampling time , while the sham group underwent laparotomy and anesthesia, the sepsis group underwent cecal ligation and puncture procedure while the vehicle group received an equivalent volume of intraperitoneal dimethyl sulfoxide (DMSO) injections for five  days followed by cecal ligation and puncture procedure, and the Atorvastatin group received intraperitoneal injections of 20 mg/kg of Atorvastatin for five days after cecal ligation and puncture procedure ,Twenty hours after the cecal ligation and puncture ,The mice were euthanized and samples of serum and cardiac tissue were collected. The levels of serum TNF-α, IL-6, and CTN-I were assessed. The data, which followed a normal distribution, was examined using t-tests and ANOVA tests with a significance level of p<0.05. The group of sepsis exhibited considerably elevated levels of TNF-α, IL-6, and CTN-I compared to the sham group. However, the pre-treated group with Atorvastatin demonstrated significantly reduced levels (p<0.05) of these markers compared to the sepsis group. The histological characteristics of mice treated with Atorvastatin showed slight variations in comparison to control and sham groups.