A Comparative Stability Study of Niosomes Prepared by Sonication Method and Ether Injection Methods

Abstract

Objective: The objective of this study is to compare the stability of Niosomes prepared using two different methods: the Sonication method and the ether injection method. By evaluating the stability parameters such as size distribution, encapsulation efficiency, and storage stability, the study aims to determine the more effective preparation technique for producing stable Niosomes.

Materials and Methods: Niosomes were prepared using two distinct methods: Sonication and ether injection. For the Sonication method, surfactants and cholesterol were dissolved in an organic solvent and then sonicated to form Niosomes. In the ether injection method, the same components were dissolved in ether and injected into an aqueous phase to form Niosomes. Both preparations were characterized for size distribution using dynamic light scattering, encapsulation efficiency using UV-visible spectrophotometer, and storage stability by monitoring changes in size and encapsulation efficiency over time at various temperatures.

Results and Discussion: The results indicated that Niosomes prepared by the Sonication method exhibited a smaller and more uniform size distribution compared to those prepared by the ether injection method. Encapsulation efficiency was found to be higher in Niosomes produced by Sonication. Over a storage period of 30 days, Niosomes from both methods showed changes in size and encapsulation efficiency; however, Niosomes prepared by the Sonication method demonstrated better stability, with less significant changes. These findings suggest that the sonication method may be more suitable for producing stable Niosomes, likely due to the more controlled and uniform size distribution it achieves.

Conclusion: In conclusion, the comparative study reveals that Niosomes prepared by the Sonication method exhibit superior stability compared to those prepared by the ether injection method. The Sonication method yields Niosomes with a smaller, more uniform size distribution and higher encapsulation efficiency, making it a preferable technique for noisome preparation in applications requiring long-term stability.