Antifungal Susceptibility Profile Of Clinical Isolates Of Candida Species And Their Resistant To Azole Compounds

Abstract

Background: Candida infections are a major health problem where the most important predisposing factors for the infections are the use of antibiotics, indwelling catheters, immunosuppression, chemotherapy and radiotherapy.

Objective:

To investigate antifungal susceptibility profile of Candida isolates toward some antifungal drugs.

Methods:

Different clinical specimens from patients suffering from candidiasis were collected. Isolation and identification of Candida spp. were done according to the laboratory standard methods. The antifungal susceptibility test for C. albicans isolate using discs diffusion method and determining the minimal inhibition concentration (MICs) were conducted to determine the values of drug resistance and  detection of some genes of resistant using specific primers and PCR method.

Results:

The isolation and identification results showed that causative agents of candidiasis  were C. albicans C. krusei C. Tropicalis C. glabrata The results of disc diffusion methods showed that the amphotericin B is the most effective drug followed by ketoconazole, fluconazole  then nystatin ,while the results of MICs showed that amphotericin B inhibited (97%),followed by ketoconazole (50%), fluconazole (31%) and nystatin (26%). The PCR assay showed the amplification of two genes related with antifungal resistant when amplify with DNA extracted from Candida isolates.

Conclusion: The study proved the emergence of drug resistant phenomena against azole agents in Candida spp.