Imbalance of Inflammatory Cytokine Response Induced by Acute Chloroform Toxicity In Vivo

Abstract

Chloroform (CH) is an environmental pollutant that induces serious health effects. This experimental study was designed to investigate the serum cytokine response induced by acute exposure to chloroform. Eighteen adult male laboratory rats were separated into three groups of eight rats each. The first control (CON) group included animals without any treatment, while the rest of the two groups were administrated trichloromethane at a dose of 477 mg/kg , orally then sacrificed after 1 day (CH-1) and 7day (CH-7) post-exposure. The results indicated that acute exposure resulted in immune disrupting through a significant increase in pro-inflammatory cytokine levels compared to unexposed control rats. In contrast, chloroform intoxication caused a remarkable decrease in the anti-inflammatory (IL-10) level in the serum of euthanized rats on 1st day after exposure. In conclusion, chloroform intoxicated caused alteration in serum inflammatory cytokine response.