The Evaluate level of heat shock protein 27 and gene expression of HSF1 in Tonsillitis Patients

Abstract

 Introduction: Tonsillitis is inflammation of the tonsils , It is more prevalent in the winter and fall seasons during school age . children with tonsillitis have their health and quality of life impacted, and it causes major illnesses, and loss of school time .

Objectives: The current study was conducted to Evaluate level of heat shock protein 27 and gene expression of heat shock factor1 in Tonsillitis sufferer .

Methods: Ninety blood sample were collected from infected people with tonsillitis attending to Tikrit Teaching Hospital in Ear ,Nose and Throat unit , tonsillitis was diagnosed depending on physical examination by specialist physician, during the period from (December 2023- March 2024 ). and Thirty sample from healthy individuals collected as a control group . each samples were splitted into two tubes ,Gel tube for the purpose of immune assay and Eppendrof containing tranZole for whole blood for purpose of RNA extraction from sample Results : Revealed that a high noteworthy difference in the serum level of heat shock protein 27 (3.185 ±0.9062) (2.495±0.7558) ng/mL respectively,(P≤0.0005), between patients and healthy controls respectively. The relative expression of HSF1 have a significant difference between patients compared to the controls (-0.761± 0.452) (7.75± 4.342) , respectively,(p₌ 0.0001).

Conclusions: difference in the concentration of heat shock protein 27(HSP27) between patients tonsillitis and healthy controls . the relative expression of HSF1 have a significant difference between patients compared to the controls (-0.7615± 0.452)( 7.975± 4.342) respectively