Evaluation of efficacy of Nelumbium Speciosum willd. seed extract on Anti-breast cancer activity in Cell line model

Abstract

The aim of the study is to evaluate the effect of Nelumbium Speciosum willd. seed extract (NSSE) in the treatment of breast cancer using MCF-7 cell lines. The phytochemical profile of the hydro-alcoholic extract (90% ethanol and 10% water) of NSSE revealed the presence of major secondary phytoconstituents. The extract was tested for its anticancer activity on the MCF-7 cell line using an MTT assay. The MTT assay showed that at a concentration of 100µg/ml, significant anticancer activity had been produced with an IC₅₀ value of 25.73µg/ml, compared to Standard 5 Fluorouracil with an IC₅₀ value of 3.17µg/ml, respectively. In the LCMS analysis, major bioactive constituents for breast cancer were identified. In silico docking studies were performed for two major compounds, namely formononetin, myricetin against two receptors, namely estrogen receptor α and estrogen receptor β receptors. Among those two compounds, formononetin  showed the strongest binding energy against all two receptors. From this, formononetin displayed the highest binding energy of -8.3kcal/mol on ER α, -7.2kcal/mol on ER β, while myricetin displayed the highest binding affinity of -7.3kcal/mol on ERα and -7.9kcal/mol on ERβ receptors. Our studies contribute to the potential of natural medicine as a viable and effective alternative. Hence, natural remedies hold great promise in the realms of healthcare and cancer therapy.