In vitro anti-oxidant and cytochrome P450 inhibitory activity of Pomegranate peel extract bio-assisted Strontium nanoparticles

Abstract

Background

Metal and metal oxidenanoparticle synthesized using extracts of plants by green synthesis technology is biologically safe, cost-effective, and environment-friendly. Plants and their extracts have established the power to devour and accumulate inorganic metal ions from their neighbouring niche. As such, green synthesis is regarded as an important tool to reduce the destructive effects associated with the physical and chemical methods of synthesis for nanoparticles commonly utilised in laboratory and industry. In light of the given background, the present study aimed to analyse the in vitroanti-oxidant and cytochrome P450 inhibitory activity of strontium nanoparticles synthesised from pomegranate peel extract.

Materials and methods

Pomegranate peel extract was prepared and strontium oxide was mixed in the extract for the synthesis of the formulated nanoparticles. The antioxidant properties of the nanoparticles were evaluated using DPPH free radical scavenging assay and ABTS radical scavenging assay with ascorbic acid as standard. Further, the cytochrome P450 inhibitory activity was determined as well under in vitro conditions.

Result

Strontium nanoparticles synthesised from Pomegranate peel extract showed significant free radical scavenging and cytochrome P450 inhibitory activities. It was observed that the percentage inhibition of ABTS radical formation and percentage inhibition of DPPH free radical formation showed dose-dependent effect along the increased concentration of nanoparticles. The CYP3A4 inhibitory activity of strontium nanoparticles showed significant increase with the increase in concentration of the nanoparticles.

Conclusion

Within the limits of the study, it can be concluded that Strontium nanoparticles synthesised from Pomegranate peel extract showed remarkable anti-oxidant and cytochrome P450 inhibitory activity. Further animal studies are needed to evaluate the efficacy of this nanocomposite.