Inhibition Of Aldolase Reductase And Protein Glycation End Product By Avocado Seed Extract Bio-Assisted Zinc Oxide Nanoparticles

Abstract

Background

Zinc oxide nanoparticles (ZnO NPs) possess a broad range of pharmacological properties, including anticancer, anti-diabetic, and antioxidant effects. Green synthesis of ZnO NPs offers enhanced bioactivity and reduced toxicity, positioning them as promising candidates for therapeutic applications. Specifically, ZnO NPs have shown potential in inhibiting key enzymes such as aldose reductase (AR) and advanced glycation end products (AGEs), which are implicated in diabetes and other health conditions.

Methods

In this study, ZnO nanoparticles were synthesized via a green chemistry approach using avocado seed extract. The inhibitory effects of the green-synthesized ZnO NPs on aldose reductase (AR) and advanced glycation end products (AGEs) were assessed using enzyme inhibition assays. The bioactivity of the nanoparticles at varying concentrations was further compared to standard drugs, quercetin and aminoguanidine to assess their potential advantages in enzyme inhibition and overall therapeutic efficacy.

Results

The green-synthesized ZnO NPs exhibited superior enzyme inhibition activity compared to conventionally synthesized ZnO NPs. At low concentrations, the green-synthesized nanoparticles showed significant inhibition of both AR and AGEs, suggesting their potential in preventing diabetic complications. Furthermore, the eco-friendly synthesis process minimized toxicity while enhancing the bioactivity of ZnO NPs, supporting their application in therapeutic interventions.

Conclusion

The green-synthesized ZnO nanoparticles demonstrated potent inhibition of AR and AGEs, suggesting their potential as a safe and effective therapeutic agent. Their eco-friendly production method further enhances their viability for use in multi-disease pathways, particularly in diabetes management.