Method Development and Validation for Concurrent Quantification of Netarsudil and Lantanoprost in Bulk and Injection form by RP-HPLC Method

Abstract

For the simultaneous determination of the dosage forms of Latanoprost and Netarsudil in ophthalmic solution, a straight forward, precise, and accurate approach was designed. Chromatogram was developed using Phenomenex C18 150 x 4.6 mm, 5m, Mobile phase containing Water: Methanol taken in the ratio 55:45 pumped through column at a flow rate of 1.0 mL/min with Potassium dihydrogen phosphate buffer at Optimized wavelength 290 nm. Temperature was maintained at 30°C.selected was. Retention time of Netarsudil and Latanoprost were found to be 2.588 min and 3.221 min. The developed method was validated by System Suitability, specificity, selectivity, accuracy, precision, accuracy, robustness and LOQ – LOD as per ICH Q2 (R1) guidelines. The regression equation of Netarsudil is y = 11999x + 1319.8 and y = 13329x + 380.64 of Latanoprost. The method developed was suitable for routine qualitative analysis of Latanoprost and Netarsudil in pharmaceutical dosage forms. Retention times were decreased, and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in industries.