Pharmacological Effect Of Ursolic Acid On Hepatocarcinoma Induced By N-Nitrosodiethylamine In Experimental Animals

Abstract

Objective: The objective of the current study was to  eavlaute the protective role of ursolic acid against N-nitrosodiethylamine (DEN) induced liver cancer in Albino Wistar rats. Materials and Methods: When rats were given 0.01% DEN in their drinking water for 15 weeks, ursolic acid had a protective effect. Aspartate transaminase (AST), alkaline phosphatase (ALP), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma glutamyltranspeptidase (γGT) are serum marker enzymes that are elevated in DEN. Lipid peroxide levels are high (in both serum and tissue), and the final body weight and tissue are subsequently reduced. To determine the antioxidant status, the amounts of GSH, SOD, LPO, and CAT as well as the activity of antioxidant enzymes were measured in the liver and hemolysate of experimental animals. By purposefully reducing these changes, ursolic acid (50 mg/kg body weight) demonstrated a potent anticancer impact in liver cancer. Results and discussion: According to the results of the biochemical estimations, DEN was successful in inducing hepatocellular carcinoma, whereas ursolic assistance was administered to counteract the effect of DEN. By evaluating different parameters of the liver tissue homogenate, the antioxidant action of ursolic acid was demonstrated. All of these findings suggest that ursolic acid has a chemopreventive impact on hepatocellular cancer caused by DEN. Conclusion: The present results suggest that Ursolic acid have protective effect by modulating the antioxidant status during DEN induced hepatocarcinogenesis.