Synthesis Of Andrographolide Containing Silver Nanoparticle For Enhancement Of Anticancer Drug Delivery

Abstract

By encasing andrographolide, which is isolated from Andrographis paniculata Nees, in silver nanoparticles, the suggested study enhances its anticancer potential. Using the Soxhlet process, andrographolide was isolated from Andrographis paniculata Nees. Its limited absorption and high hydrophobicity limit its clinical use as a chemopreventative drug. In order to improve solubility, absorption, and affectivity against tumours, its silver nanoparticles were created. The formation of nanoparticles was monitored using a variety of analytical techniques and assays, including drug release, haemolytic toxicity study, in vitro cytotoxicity study, zeta potential, particle size, Atomic Force Microscopy (AFM), Differential Scanning Colorimetric analysis (DSC), X-ray diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR). Atomic force microscopy and particle size analysis showed the presence of silver nanoparticles with an average size of 17.98 nm, and a zeta potential of -4.68 mV was determined. An-AgNPs were able to release 95.11% of the andrographolide in 12 hours, while the andrographolide was released 94.21% in 4 hours. The results of haemolytic toxicity showed that An-AgNPs exhibits less hemotoxicity than plain andrographolide. X-ray diffraction was used to determine the synthesised nanoparticles' crystalline nature. According to the findings of the invitro cytotoxicity investigation, andrographolide-induced An-AgNPs cytotoxicity to the tumour cells was dosage dependant and more cytotoxic than that of plain andrographolide. This study demonstrates the potential anti-cancer effects of silver nanoparticles encapsulated in andrographolide.