Molecular study of Diplostomum spathaceum (Rud.1819) (Digenea) parastic the fish eyes of Carasobarbus luteus and Cyprinus carpio Southern of Iraq

Abstract

Background: The larval stage (metacercariae) of Diplostomum spp. is regarded as a causative agent  of the worm cataract diplostomiasis is many freshwater fishes  of Iraq.The ami of the current paper was  to study the molecular diagnosis of Diplostomum Parasite  isolate from the eyes of the  Carasobarbus  luteus  and Cyprinus carpio using  ribosomal 28s rDNA gene amplified  by PCR technique. Methods: Atotal of (236) Carasobarbus luteus and (92) Cyprinus carpio fishes  were collected from Thi Qar and Basrah Governorates  during the period from 1/6 to 30/12/2022.. The live fish were transferred in a cork containing a quantity of river water to the Laboratory College of Education for Pure Sciences at Thi Qar  and Basrah Universities .PCR technique ,specific gene and  primers was used . Results: The polymerase chain reaction (PCR) technique, using the gene R 1500, showed the appearance of bands at the 1300 bp site in all DNA samples .  The sequence of the two local isolates Lc739726.1 and Lc739727.1 of the D. spathaceum parasite isolated from the eyes of the C. Luteus fish showed closeness with the sequence of the two Czech isolates KR269765.1 and the Ukrainian isolate MZ314171 with a 100% match. Conclusion: Two local of Diplostomum spathaceum isolates LC739726.1 , LC739727.1 and one local isolate of Diplostomum SP.AA1gene LC768865 found in the present study from C.luteus and C.carpio respectively.