Antioxidant, Anti-Inflammatory and Anticancer Activity of Nigella Sativa and Terminalia Chebula

Abstract

Aims: The selected herbs treat many diseases, as per folkmedicinal data. Nowadays, due to the food habits of this generation, they are easily prone to various diseases and infections. This present study aims to study the anticancer activity (Colon cancer cells) of the selected powdered skin of Terminalia chebula and the powdered Nigella sativa extracts since the selected components are traditionally used to treat many diseases.

Settings and Design: The extract prepared separately from the powdered skin of Terminalia chebula and the powdered Nigella sativa. The individual extracts were studied for the anti-cancer activity in the basis of 1:1 ratio in the colon cancer cells.

Methods and Material: The following studies were performed, which are as follows: FT-IR, GC-MS, to confirm the presence of chemical constituents present in the prepared extracts. DPPH Assay for Antioxidant activity, Anti-inflammatory Assay, and MTT Assay in colon cancer cell lines performed to study for anticancer activity.

Results: The presence of Thymoquinone, cyclohexasiloxanedodecamethyl from the Nigellea sativa, and Terminalia chebularepectively using FT-IR & GC-MS analysis. The prepared extracts exhibit antioxidant activity at the concentration of 80 μg/ml using DPPH assay.The protein denaturation analysis proved the prepared extracts showed anti-inflammatory activity at a concentration of80 μg/ml and MTT assay showed the better results of the prepared extracts in 1:1 ration at  90.90μg/ml.

Conclusions: Based on the results the prepared extracts are promising chemotherapeutic agent in treating colon cancer cell. This study helps to develop the promising dosage forms in treating colon cancer cells.